
pBABEhygro逆病毒载体质粒
产品名称: pBABEhygro逆病毒载体质粒
英文名称: pBABEhygro逆病毒载体质粒
产品编号: BiovectorPBABEhygro
产品价格: 0
产品产地: Biovector NTCC Inc. USA
品牌商标: Biovector,NTCC, Addgene, ATCC,
更新时间: null
使用范围: null
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
- 电话 : 189****8599 点击查看
- 传真 : 点击查看
- 邮箱 : Biovector@163.com
Product Information
Order ID |
Name |
Description |
BiovectorpBABEhygro |
pBABEhygro |
pBABEhygro,DNA. 15uL,AmpR.Storage:-20℃ |
Transformation of plasmid DNA to competent E. Coli cells
- Thaw competent cells on ice. 20–200µL per tube
- Add max. 1-3µL of plasmid
- Mix very gently!
- Incubate the tubes on ice for 30 min
- Heat shock the cells for 45 sec to 2 min at 42°C
- Place the tubes immediately on ice for at least 2 min
- Add 800µL of SOC medium to each tube
- Incubate for 1 hour at 37°C and shake vigorously
- Spin down briefly and remove most supernatants
- Resuspend cell pellet with the rest SOC medium in the tube by pipetting
- Plate out the suspension on a LB agar plate containing the appropriate antibiotic. Incubate the plates overnight at 37°C
Growth in Bacteria
· Bacterial Resistance(s)
Ampicillin
· Growth Temperature
37°C
· Growth Strain(s)
DH5alpha
· Copy number
High Copy
>sequence 5558bp
CTGCAGCCTGAATATGGGCCAAACAGGATATCTGTGGTAAGCAG
TTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGAACAGCTGAAT
ATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGC
TCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCAGCCCTCA
GCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGG
ACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTC
GCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATA
AAAGAGCCCACAACCCCTCACTCGGGGCGCCAGTCCTCCGATTG
ACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTTGCA
GTTGCATCCGACTTGTGGTCTCGCTGTTCCTTGGGAGGGTCTCC
TCTGAGTGATTGACTACCCGTCAGCGGGGGTCTTTCATTTGGGG
GCTCGTCCGGGATCGGGAGACCCCTGCCCAGGGACCACCGACCC
ACCACCGGGAGGTAAGCTGGCCAGCAACTTATCTGTGTCTGTCC
GATTGTCTAGTGTCTATGACTGATTTTATGCGCCTGCGTCGGTA
CTAGTTAGCTAACTAGCTCTGTATCTGGCGGACCCGTGGTGGAA
CTGACGAGTTCTGAACACCCGGCCGCAACCCTGGGAGACGTCCC
AGGGACTTTGGGGGCCGTTTTTGTGGCCCGACCTGAGGAAGGGA
GTCGATGTGGAATCCGACCCCGTCAGGATATGTGGTTCTGGTAG
GAGACGAGAACCTAAAACAGTTCCCGCCTCCGTCTGAATTTTTG
CTTTCGGTTTGGAACCGAAGCCGCGCGTCTTGTCTGCTGCAGCA
TCGTTCTGTGTTGTCTCTGTCTGACTGTGTTTCTGTATTTGTCT
GAAAATTAGGGCCAGACTGTTACCACTCCCTTAAGTTTGACCTT
AGATCACTGGAAAGATGTCGAGCGGCTCGCTCACAACCAGTCGG
TAGATGTCAAGAAGAGACGTTGGGTTACCTTCTGCTCTGCAGAA
TGGCCAACCTTTAACGTCGGATGGCCGCGAGACGGCACCTTTAAC
CGAGACCTCATCACCCAGGTTAAGATCAAGGTCTTTTCACCTGGC
CCGCATGGACACCCAGACCAGGTCCCCTACATCGTGACCTGGGAA
GCCTTGGCTTTTGACCCCCCTCCCTGGGTCAAGCCCTTTGTACAC
CCTAAGCCTCCGCCTCCTCTTCTTCCATCCGCGCCGTCTCTCCCC
CTTGAACCTCCTCTTTCGACCCCGCCTCAATCCTCCCTTTATCCA
GCCCTCACTCCTTCTCTAGGCGCCGGCCGGATCCCAGTGTGGTGG
TACGTAGGAATTCGCCAGCACAGTGGTCGACCCTGTGGAATGTGT
GTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAA
GTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAA
AGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATC
TCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCC
CGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCT
GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCT
CTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAG
GCTTTTGCAAAAAGCTTACCATGACCGAGTACAAGCCCATGAAAA
AGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAA
AGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAG
AATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCC
TGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATG
TTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGC
TTGACATTGGGGAGTTCAGCGAGAGCCTGACCTATTGCATCTCCC
GCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAAC
TGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCG
CTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGAC
CGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCG
CGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACG
ACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGC
TTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGG
ATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAG
CGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACG
AGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGG
AGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAG
GATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACC
AACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTT
GGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGA
CTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGA
CCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCC
CCAGCACTCGTCCGAGGGCAAAGGAATATCGATAAAATAAAAGAT
TTTATTTAGTCTCCAGAAAAAGGGGGGAATGAAAGACCCCACCTG
TAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATG
GAAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTCAGG
AACAGATGGAACAGCTGAATATGGGCCAAACAGGATATCTGTGGT
AAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGAACAGC
TGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCC
CGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCAGCCC
TCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAA
GGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTT
CGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATA
AAAGAGCCCACAACCCCTCACTCGGGGCGCCAGTCCTCCGATTGA
CTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTTGCAGT
TGCATCCGACTTGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCT
GAGTGATTGACTACCCGTCAGCGGGGGTCTTTCATTTCCGACTTG
TGGTCTCGCTGCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACC
CGTCAGCGGGGGTCTTCACATGCAGCATGTATCAAAATTAATTTG
GTTTTTTTTCTTAAGTATTTACATTAAATGGCCATAGTTGCATTA
TUNEology 波长可调检测卡盒-->