PTEN polyclonal antibody
产品名称： PTEN polyclonal antibody
英文名称： PTEN polyclonal antibody
产品编号： PAB12725
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PTEN.
- Immunogen:
- A synthetic peptide corresponding to residues surrounding S385 of human PTEN.
- Host:
- Rabbit
- Reactivity:
- Bovine, Chicken, Human, Mouse, Rat
- Specificity:
- This antibody recognizes PTEN (Paired S385) without a phosphorylation site at Serine 385. It does not cross-react with phosphospecific peptides.
- Form:
- Liquid
- Storage Buffer:
- In TBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:200)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.
Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T.J Biol Chem. 2005 Oct 21;280(42):35195-202. Epub 2005 Aug 17.
- Applications
- Western Blot (Tissue lysate)
- The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN polyclonal antibody (Cat # PAB12725) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (Lane 2).
- Dot Blot (Peptide)
- Dot Blot : 1 ug peptide was blot onto NC membrane.
A : PTEN (pS385) (Phosphospecific)
B : PTEN (Paired S385) (Non phosphospeicfic)
C : Non-related phosphospecific peptide
were blotted at a 1 : 2000 dilution by :
1 : Phospho-PTEN S385 polyclonal antibody (Cat # PAB12751),
2 : PTEN polyclonal antibody (Cat # PAB12725).
- Entrez GeneID:
- 5728
- Gene Name:
- PTEN
- Gene Alias:
- 10q23del,BZS,MGC11227,MHAM,MMAC1,PTEN1,TEP1
- Gene Description:
- phosphatase and tensin homolog
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq
- Other Designations:
- MMAC1 phosphatase and tensin homolog deleted on chromosome 10,OTTHUMP00000020032,mutated in multiple advanced cancers 1
- Related Disease
- Abnormalities, Multiple
- Adenocarcinoma
- Adenocarcinoma, Papillary
- Alzheimer Disease
- Alzheimer disease
- Astrocytoma
- Autistic Disorder
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma, Endometrioid
- Carcinoma, Squamous Cell
- Cardiovascular Diseases
- Cleft Lip
- Cleft Palate
- Colon cancer
- Colonic Neoplasms
- Colorectal Neoplasms
- Craniofacial Abnormalities