Description

Schwann cells are neural crest derivatives that ensheathe and myelinate axons of peripheral nerves [1]. They wrap individually around the shaft of peripheral axons, forming a layer or myelin sheath along segments of the axon. Schwann cells play important roles in the development, function and regeneration of peripheral nerves. When an axon is dying, the Schwann cells surrounding it aid in its digestion. This leaves an empty channel formed by successive Schwann cells, through which a new axon may grow from a severed end. The number of Schwann cells in peripheral nerve is tightly regulated [2]. Their proliferation in vitro can be stimulated by polypeptide growth factors including PDGF, FGF, neuregulin and others [3]. The Schwann cells provide a relatively simple, well-defined and accessible mammalian model for the study of a number of developmental questions. It is also of particular clinical importance to understand the biology of Schwann cells, not only in the context of neuropathies and nerve regeneration, but also because the cells or their precursor might be especially well suited as implants to facilitate repair in the CNS.

MSC from ScienCell Research Laboratories are isolated from mouse sciatic nerves. MSC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MSC are characterized by immunofluorescent method with antibodies to S-100, GFAP and CD90. MSC are negative for mycoplasma, bacteria, yeast and fungi. MSC are guaranteed to further expand for 10 population doublings in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Schwann Cell Medium (SCM, Cat. No. 1701) for the culturing of mouse Schwann cells in vitro.

Product Use

MSC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

（三）．小鼠正常细胞
    M1200 小鼠垂体细胞（MPC）（5×105）
    M1400 小鼠脑膜细胞（MMC）（5×105）
    M1510 小鼠中脑缝神经细胞(脑脊)（MN-r）（1×106）
    M1520 小鼠神经黑质细胞（MN-sn）（1×106）
     M1530 小鼠条纹神经细胞（MN-s）（1×106）
     M1540 小鼠海马趾神经细胞（MH-h）（1×106）
     M1550 小鼠神经皮质细胞（MN-c）（1×106）
     M1560 小鼠颗粒神经元（MGC）（1×106）
     M1570 小鼠腹脊髓神经细胞（MN-vsc）（1×106）
     M1580 小鼠背部脊髓神经元（MN-dsc）（1×106）
    M1600 小鼠少突胶质前体细胞(MOPC)(5×105)
    M1700 小鼠雪旺细胞（MSC）（5×105）
    M1710 小鼠周神经成纤维细胞(MPNF)(5×105)
    M1800 小鼠星形胶质细胞（MA）（5×105）
     M1810 小鼠小脑星形胶质细胞（MA-c）（5×105）
     M1820 小鼠海马趾星形胶质细胞（MA-h）（5×105）
     M1900 小鼠小神经胶质细胞（MM）（5×105）
     M2530 小鼠淋巴纤维细胞（MLF）（5×105）
     M4100 小鼠肾脏上皮细胞（MREpiC）（5×105）
     M6200 小鼠心肌细胞（MCM）（1×106）
     M7500 小鼠骨髓间充质肝细胞（MMSC-bm）（5×105）
    M7540 小鼠胚胎成纤维细胞(MEF)(5×105)
    M7550 小鼠胚胎成纤维细胞(MEF-mt)(5×105)