Uniprot ID:P56255
Gene Names:pcrA
Source:E.coli
Tag Info:His-SUMO-tag
Expression Region:10-289aa
MW:48.57kD
Sequence Info:Full length of ATP-binding Domain
Alternative name(s):
relevance:DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.
reference:"Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism."
Bird L.E., Brannigan J.A., Subramanya H.S., Wigley D.B.
Nucleic Acids Res. 26:2686-2693(1998) 
Purity:Greater than 90% as determined by SDS-PAGE.
Storage Buffer:20mM Tris-HCl based buffer,pH8.0
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
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