MAP2K1/MAP2K2 polyclonal antibody
产品名称： MAP2K1/MAP2K2 polyclonal antibody
英文名称： MAP2K1/MAP2K2 polyclonal antibody
产品编号： PAB12707
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MAP2K1/MAP2K2.
- Immunogen:
- A synthetic peptide corresponding to residues surrounding S218 and S222 of human MAP2K1/MAP2K2.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 44, 45
- Reactivity:
- Chicken, Human, Mouse, Rat
- Specificity:
- This antibody recognizes ~44, 45 KDa of human MAP2K1 without phosphorylation sites of Ser 218/222 or MAP2K2 (pS222/226). It does not cross react to phospho MAP2K1/MAP2K2.
- Form:
- Liquid
- Storage Buffer:
- In TBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Inhibition of the Raf-MEK1/2-ERK1/2 signaling pathway, Bcl-xL down-regulation, and chemosensitization of non-Hodgkin's lymphoma B cells by Rituximab.
Jazirehi AR, Vega MI, Chatterjee D, Goodglick L, Bonavida B.Cancer Res. 2004 Oct 1;64(19):7117-26.
- Applications
- Western Blot (Cell lysate)
- The whole cell lysates derived from NIH/3T3 were immunoblotted by MAP2K1/MAP2K2 polyclonal antibody (Cat # PAB12707) at 1 : 1000.
- Dot Blot (Peptide)
- Dot Blot : 1 ug peptide was blot onto NC membrane.
A : MEK1/2 (pS218/222).
B : MEK1/2 (Non-phospho).
C : Non-related phospho-specific phosphopeptide.
Followed were blotted at a 1 : 1000 dilution by :
1. Phospho-MAP2K1/MAP2K2 S218/S222 polyclonal antibody (Cat # PAB12635).
2. MAP2K1/MAP2K2 polyclonal antibody (Cat # PAB12707).
- Entrez GeneID:
- 5604
- Gene Name:
- MAP2K1
- Gene Alias:
- MAPKK1,MEK1,MKK1,PRKMK1
- Gene Description:
- mitogen-activated protein kinase kinase 1
- Omim ID:
- 176872
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
- Other Designations:
- protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
- Entrez GeneID:
- 5605
- Gene Name:
- MAP2K2
- Gene Alias:
- FLJ26075,MAPKK2,MEK2,MKK2,PRKMK2
- Gene Description:
- mitogen-activated protein kinase kinase 2
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq
- Other Designations:
- ERK activator kinase 2,MAP kinase kinase 2,MAPK/ERK kinase 2,dual specificity mitogen-activated protein kinase kinase 2,mitogen-activated protein kinase kinase 2, p45