PHB (N-term) Peptide
产品名称： PHB (N-term) Peptide
英文名称： PHB (N-term) Peptide
产品编号： P1194
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PHB.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 99-129 at N-terminus of human PHB.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Prohibitin silencing reverses stabilization of mitochondrial integrity and chemoresistance in ovarian cancer cells by increasing their sensitivity to apoptosis.
Gregory-Bass RC, Olatinwo M, Xu W, Matthews R, Stiles JK, Thomas K, Liu D, Tsang B, Thompson WE.Int J Cancer. 2008 May 1;122(9):1923-30.
- 2.
- The PHB1/2 phosphocomplex is required for mitochondrial homeostasis and survival of human T cells.
Ross JA, Nagy ZS, Kirken RA.J Biol Chem. 2008 Feb 22;283(8):4699-713. Epub 2007 Dec 17.
- 3.
- Assignment of the human prohibitin gene (PHB) to chromosome 17 and identification of a DNA polymorphism.
White JJ, Ledbetter DH, Eddy RL Jr, Shows TB, Stewart DA, Nuell MJ, Friedman V, Wood CM, Owens GA, McClung JK, et al..Genomics. 1991 Sep;11(1):228-30.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of PHB1 (arrow) using rabbit PHB polyclonal antibody (Cat # PAB2547). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB1 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with PHB polyclonal antibody (Cat # PAB2547), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 5245
- Protein Accession#:
- NP_002625;P35232
- Gene Name:
- PHB
- Gene Alias:
- PHB1
- Gene Description:
- prohibitin
- Omim ID:
- 176705
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq
- Other Designations:
- -