PKN1 polyclonal antibody
产品名称： PKN1 polyclonal antibody
英文名称： PKN1 polyclonal antibody
产品编号： PAB2308
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PKN1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PKN1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family.
Palmer RH, Ridden J, Parker PJ.Eur J Biochem. 1995 Jan 15;227(1-2):344-51.
- 2.
- Identification of multiple, novel, protein kinase C-related gene products.
Palmer RH, Ridden J, Parker PJ.FEBS Lett. 1994 Dec 12;356(1):5-8.
- 3.
- A novel protein kinase with leucine zipper-like sequences: its catalytic domain is highly homologous to that of protein kinase C.
Mukai H, Ono Y.Biochem Biophys Res Commun. 1994 Mar 15;199(2):897-904.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PKN1 polyclonal antibody (Cat # PAB2308) in HeLa cell lysate. PKN1 was detected at 104 kDa (arrow) using purified polyclonal antibody. A protein of unknown identity was also detected at 60 kDa. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PKN1 polyclonal antibody (Cat # PAB2308) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 5585
- Protein Accession#:
- Q16512;acc50209
- Gene Name:
- PKN1
- Gene Alias:
- DBK,MGC46204,PAK1,PKN,PKN-ALPHA,PRK1,PRKCL1
- Gene Description:
- protein kinase N1
- Omim ID:
- 601032
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
- Other Designations:
- protein kinase C-like 1,protein kinase C-like PKN,protein kinase C-related kinase 1,serine-threonine kinase N,serine/threonine protein kinase N
- Related Disease