Golden 1st cDNA Synthesis Kit
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| cDNA第一链反转录试剂盒采用稳定高效的反转录预混体系5×Golden RT MasterMix进行RNA的反转录反应，使用时只需加入模板、引物和RNase Free H2O 即可，大大简化了操作过程、提高了效率、减少了操作过程中的人为误差。MasterMix中添加了HL dsDNase（热敏双链特异性核酸酶），该双链特异性核酸酶（又名gDNA Remover）可在反转录的过程中，直接降解去除RNA中残留的基因组DNA污染(可去除100ng基因组DNA)，从而在定量PCR的检测中无需考虑基因组污染的干扰，设计的定量PCR引物也无需进行横跨外显子。该试剂盒尤其适用于定量PCR检测。 该预混体系包含点突变致RNase H活性缺失的Golden MLV Reverse Transcriptase反转录酶、dNTP、反应Buffer、gDNA去除试剂和RNase Inhibitor。该试剂盒采用的反转录酶去除了RNase H活性，从而避免反转录过程中降解RNA。同时经过突变文库筛选，使得其热稳定性更强，可耐受55℃高温反应。相比于低温条件下反转录反应，采用高温反转录可显著打开RNA二级结构，从而提高复杂RNA模板的扩增性能、提高反转录cDNA的长度和产量，从而提高后续检测的灵敏度。合成的第一链cDNA可广泛用于2nd Strand的合成、杂交、PCR扩增、Real-Time PCR反应等。 |
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| 注：1.该制品可有效反转录mRNA、tRNA、LncRNA、ncRNA。 2. 该制品不可反转录microRNA、病毒DNA/RNA样品。 |
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| 请置于-20°C，可保存2年；避免反复冻融。注：如5×Golden RT MasterMix(with dsDNase)出现沉淀属正常现象，可用手捂化，混合均匀后使用不影响实验结果。 | ||||||||||||||||||||||
应用本产品文献 |
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